By Verena Beier, Cora Mund, Jörg D. Hoheisel (auth.), Harald Seitz (eds.)
Analytics of Protein-DNA Interactions covers developments in sleek biotechnology. All features of this interdisciplinary expertise, the place wisdom, equipment and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and desktop technology, are handled. additional information in addition to the digital model is out there at springer.com.
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Extra info for Analytics of Protein–DNA Interactions
This criterion, as well as the simulation of the response based on the evaluated rate constant (smooth lines in Fig. 5a), validates the analysis. 1 Introduction Contrary to gel retardation or analytical ultracentrifugation techniques, where every DNA · PROTEIN species can be separated and quantitatively analyzed, the response curve in SPR experiments represents the sum of all binding events between protein and DNA containing multiple binding sites. For this reason quantitative analysis of protein binding to more then one binding site on DNA is relatively difﬁcult, and successful only when binding events differ signiﬁcantly in kinetic constants.
Paralogous Genes Can Diverge in Coding Potential and Expression Pattern Molecular Changes in Paralogous Gene Regulation . . . . . . . Recently Duplicated Genes . . . . . . . . . . . . . . The Epidermal Differentiation Complex (EDC) . . . . . . . . CE Precursor Genes and “Fused Genes” . . . . . . . . . . 1 . 3 Proof-of-Principle: Novel Elements in the SPRR3 Promoter . . . . Identiﬁcation of SPRR3-Speciﬁc Regulatory Elements in the Proximal Promoter .
Reported recently the development of an Au nanocluster embedded in a dielectric ﬁlm to achieve a tenfold improvement in sensitivity . Presently SPR is used to study individual protein–DNA interactions with respect to kinetics, thermodynamics , or stoichiometry. One important development in the future will be to detect simultaneously a large number of interactions in parallel. BIAcore recognized this demand and recently introduced the Flexchip, which uses a sensor chip that allows simultaneous analysis of 400 interactions on a single biosensor, however with a slightly reduced sensitivity.
Analytics of Protein–DNA Interactions by Verena Beier, Cora Mund, Jörg D. Hoheisel (auth.), Harald Seitz (eds.)