Download PDF by Robert O. Kelley, Kathryn G. Vogel (auth.), John E. Johnson: Aging and Cell Structure: Volume 2

By Robert O. Kelley, Kathryn G. Vogel (auth.), John E. Johnson Jr. (eds.)

ISBN-10: 146844607X

ISBN-13: 9781468446074

ISBN-10: 1468446096

ISBN-13: 9781468446098

Although hundreds of thousands of articles and hundreds of thousands of books on getting older were released, just a small percent of this fabric has handled anatomy, quite on the tremendous­ structural point. It used to be with this in brain that getting older and phone constitution used to be conceived. quantity 1 of getting older and mobilephone constitution used to be released in 1981 and represented a present compilation of knowledge, concentrating on the electron microscopic point, on morphological adjustments which happen in cells and tissues as they age. the current quantity completes the two-volume set. whereas quantity 1 highlighted structural adjustments happening within the getting older anxious approach, quantity 2 facilities its efforts on reviews of in vitro getting older. Chapters on different matters are integrated in addition. those comprise age-related alterations visible in neuromuscular junctions, oral tissues, and the pancreas. even if those volumes characterize a really small a part of the printed infor­ mation on experimental gerontology, their process is quite precise simply because they specialize in anatomy, possibly the main easy of the entire biomedical sciences. simply because many dif­ ferent tissue varieties are tested, we commence to work out recurrent, definitive styles within the getting older phone which could now not be totally obvious from experiences taking one cellphone style at a time. This turns into much more obtrusive within the current quantity the place adjustments noticeable in popula­ tions of cells grown in culture-isolated from hormones or worried impulses from different physique areas-are discovered to be just like these alterations present in vivo.

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By indirect immunofluorescence (Fig. , 1981a). , 1980c). Fibronectin released to 19 20 ROBERT O. KELLEY and KA THYRN G. VOGEL Figure 9. Indirect immunofluorescence microscopy of lung fibroblast cultures at different population doubling levels and stained for fibronectin. (a) PDL 16; (b) PDL 53: Internal fluorescence of late-passage cells was yellow in color and clearly distinct from the FITC-Iabeled second antibody. Note absence of fibrillar fibronectin over large, late-passage cells. X 1000. the medium by cell cultures could be purified on a collagen-Sepharose affinity column, and this material demonstrated the usual band position by SDS-polyacrylamide gel electrophoresis (Chandrasekhar and Millis, 1978).

At that time, of course, electron microscopy was not available. " Collier (1943) described them in pineals of mice and rats. Quay (1965) did not see them in rodents but concluded that their occurrence, in general, may reflect increased cell activity. Wolfe (1965), using electron microscopy, reported the presence of pinealocyte nuclear inclusions in specimens fixed by immersion. Kevorkian and Wessel (1959), also utilizing electron microscopy, failed to find nuclear inclusions in the human pineal, and concluded that previous light microscopic reports of their presence were based on misconstrued folds in the nucleus.

Rev. Biophys. 7:527. , 1968, Biochemistry of the cell cycle: A review, Cell Tissue Kinet. 1:167. , Marek, L. , Levinstone, D. , Young, I. , Science 202:1158. THE AGING CELL SURFACE Bennett, M. V. , 1973, Functions of electrotonic junctions in embryonic and adult tissues, Fed. Proc. 32:65. , 1976, Vertical displacement of membrane proteins mediated by changes in microviscosity, Proc. Natl. Acad. Sci. USA 73:4526. Bosmann, H. , Gutheil, R. , and Case, K. , 1976, Loss of a critical neutral protease in ageing WI-38 cells, Nature 261:499.

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Aging and Cell Structure: Volume 2 by Robert O. Kelley, Kathryn G. Vogel (auth.), John E. Johnson Jr. (eds.)

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